Researchers in Sweden and Finland have established a new type of food testing method that can quickly and accurately probe pathogens carried by food, which will provide effective help for food safety supervision. The related papers were published in the recently published Journal of Applied and Environmental Microbiology.
The researchers used the TaqMan probe real-time quantitative PCR (polymerase chain reaction) method to detect Yersinia enterocolitica in food, and tested the detection results in a variety of ways, such as using this method to artificially cultivated Samples containing milk, minced meat, cold smoked meat, fish and carrots containing related pathogens were tested. The test results showed that the TaqMan probe method can detect bacteria contained in foods sensitively, stably, accurately, and efficiently, which will become The effective means of food safety supervision.
Yersinia enterocolitica is a major cause of intestinal disease in Yersinia pestis. The disease is similar to appendicitis and can cause diarrhea, high fever, abdominal pain, and vomiting. Many people think that living pigs are the main sites for Y. enterocolitica, and pork is the most likely route to transmitting the bacteria to the human body. Yersinia enterocolitica can reproduce at low temperatures and under vacuum conditions, which raises concerns about food safety. Today's germ detection methods are time-consuming and inefficient, and cannot rapidly detect pathogens in food.
Researchers said that the establishment of a rapid, accurate, and specific new detection method can replace the existing detection methods of food bacteria, in order to protect food safety and avoid the risk of food disease pave the way.
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