Prochloraz is an imidazole fungicide . In recent years, it has achieved good control effect against rice seedling disease, but at present some local areas have also experienced a decline in control effect. Mole seedling disease is an important disease on rice. The benzoxazole drug represented by carbendazim was the main agent for the prevention and treatment of this disease. With the resistance of the pathogen to the carbendazim, the drug is used alone. The effect is declining.
In this regard, College of Agronomy and Biotechnology, Ministry of Agriculture pesticide test the China Agricultural University researchers using mycelium growth rate and microscopic observation to study the strain prochloraz on rice seedlings evil disease bacteria strains sensitive and resistant mutants growth Effects of development and mycelial growth morphology. The results showed that: 0.5 mg / liter of prochloraz solution can completely inhibit the mycelial growth of sensitive strains, can not inhibit the mycelial growth of resistant strains with different resistance levels; 20 mg / liter of prochloraz solution can completely inhibit the parents The spore germination of sensitive strains inhibited the spore germination and germ tube elongation of different resistant mutants by 84.1%-89% and 58.5%-65.8%, respectively, indicating that the resistant mutant strains grew in mycelium, spore germination, The sensitivity to the agent at the stage of elongation of the germ tube is reduced to a different extent than that of the parental sensitive strain. After treating the hyphae with 10 mg/L of prochloraz solution for 12 hours, the hyphae of the parental sensitive strain expanded and deformed, and the hyphal top of the resistant mutant expanded, but there were many small branches on the hyphae, indicating that the agent was antagonistic. The inhibitory effect of the secondary mutant hyphae of the mutant was not obvious.
The above test results indicate that the rice seedling pathogen has potential drug resistance risk to prochloraz, and it is recommended to rotate the drug with a different mechanism of action such as dithiocyanomethane or trichloroisocyanuric acid to delay or Avoid drug resistance.
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